ABMG028
DNase I from ABM, 2,000 Units (1.0 ml)

DNase I from ABM, 2,000 Units (1.0 ml)

Features

• Concentration: 2U/µl
• Composition: Enzyme supplied with 10X Reaction Buffer
• Storage: 10 mM Tris-HCl (pH 7.6), 2 mM CaCl₂, and 50% (v/v) Glycerol.
• Handling: Store all components at -20° C

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 µg of DNA in 10 minutes at 37° C into tetranucleotides or smaller fragments.

Application

• Removes DNA from protein preparations and RNA samples
• Mediates nick translation
• Generates random fragments for dideoxy sequencing
• Degrade template DNA following in vitro transcription
• Mediate DNase I foot-printing

Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.

This product is distributed for laboratory research only. Caution: not for diagnostic use.

Protocl  QC Document