ABMG251
Ni-IDA Agarose Resins for Protein Purification from ABM, 25 ml

Ni-IDA Agarose Resins for Protein Purification from ABM, 25 ml

Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni²⁺ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.

Applications

Purification of His-tagged proteins.

Antibody purification.

Assays involving protein-protein and protein-DNA interactions.

Structural and functional investigation of proteins.

Special Features

One-step easy purification protocol from crude lysate to >95% pure protein.

Purification under native or denaturing conditions.

Ready-to-use for any scale of purification.

Protein binding capacity: approx. 20-40 μmol Ni²⁺/ml gel.

Economical value.

Can be used for successive cycles.

Resins and columns formats suitable for batch and gravity purification.