Electrophoresis Buffers

Electrophoresis Buffers

Features:

• Formulated with 18 Megohm water and 0.2 Micron filtration
• More economical than benchtop-made buffers
• Saves time and improves results
• Suitable for small proteins
• Costs less than bench-made buffers
• Certified RNase and DNase free

TTE Glycerol Tolerant Buffer (20X)

TTE Glycerol Tolerant Buffer (20X) is a concentrated Tris-Taurine-EDTA buffer solution in distilled/deionized water. TTE (20X) eliminates the band distrotio associated with DNA samples in glycerol. TTE Glycerol Tolerant Buffer (20X) is supplied in 1 liter bottles, containing 1.78M Tris base, 0.57M Taurine and 10 mM Na₂EDTA.

TAE Buffer (50X)

TAE Buffer (50X) is a concentrated solution fo 2M Tris-acetate and 100 mM NA₂EDTA in distilled/deionized water (pH 8.3 at 1X concentration). TAE Buffer is used for agarose DNA electrophoresis.
Tris-Tricine-SDS PAGE Buffer (10X)

Discontinuous SDS-PAGE employing Tris-Glycine as the tank buffer resolves proteins down to about 15 kd, however, below this size, the proteins do not destack from the sDS micelles running through the gel with the buffer front, In order to resolve proteins in this size range, the Tris-Tricine system of Schagger and von Jagow (1987) was developed. An alternative to the Schagger and von Jagow System involves running Tris-Tricine Gels using 10X Tris-Tricine-SDS Buffer. With Tris-Tricine-SDS, you can extend the range of SDS-PAGE to resolve smaller proteins with minimal alteration of protocol.

To provide this level of convenience, the original method of Shaggar and Von Jagow (1987 Anal Biochem 166) was streamlined by develloping Tris-Tricine-SDS cathode tank buffer to be compatible with the standard Laemmli gel/buffer system. This combination resolves proteins as small as 5kD. The researcher simply substitutes Tris-Tricine-SDS in the upper (cathode) tank, with no other changes from standard Laemmli protocol to extend the resolution of their gels.

TBE Buffer & TBE Buffer (5X)

TBE Buffer is a concentrated buffer solution of Tris-Borate-EDTA in distilled/deionized water. TBE is ready-to-use as a 10X concentrate. When diluted, the 1X solution contains 0.089M Tris base, 0.089M boric acid (pH 8.3) and 2 mM Na₂EDTA.

Protein Loading Buffer Blue (2X)

AquaPor Protein Loading Buffer Blue is a ready-to-use buffer solution for the preparation of protein samples to be separated in SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Loading Buffer Blue (2X) contains Bromophenol Blue as a tracking dye to monitor the sample during the electrophoretic run. Loading Buffer Blue (2X) contains 0.5M Tris-HCl (pH 6.8), 4.4% (w/v) SDS, 20% (v/v) glycerol, 2% (v/v) 2-mercaptoethanol, and bromophenol blue in distilled/deionized water.

Tris-Glycine Electroblotting Buffer (10X)

Tris-Glycine Electroblotting Buffer (10X) is a concentrated solution in distilled/deionized water. This buffer is intended to act as the thank buffer in Western electroblotting procedures. It is also the recommended buffer for use with nitrocellulose and nylon membranes. Tris-Glycine Electroblotting Buffer (10X) is already in solution which eliminates the weighing, mixing and adjusting of the pH necessary with powdered buffers. To prepare 1 liter of working strength buffer, add 100 ml of Tris-Glycine Electroblotting Buffer to 200 ml of methanol and 700 ml of distilled/deionized water.
Tris-Glycine Electroblotting Buffer (10X) contains 0.25M Tris base and 1.92M glycine, with a pH of 8.4.

Available Models

Product Code	Description
NDIEC-860-1	TBE Buffer (10X), 1 Litre, Each
NDIEC-860-4	TBE Buffer (10X), 4 Litre, Each
NDIEC-869	Tris-Tricine-SDS Page Buffer (10X), Each
NDIEC-870	Tris-Glycine-SDS Page Buffer, 1 Litre, Each
NDIEC-870-4	Tris-Glycine-SDS Page Buffer, 4 Litre, Each
NDIEC-871	TTE Glycerol Tolerant Buffer (20X), 1 Litre, Each
NDIEC-872	TAE Buffer (50X), Each
NDIEC-880-1	Tris-Glycine Electroblotting Buffer (10X), 1 Litre, Each
NDIEC-880-4	Tris-Glycine Electroblotting Buffer (10X), 4 Litre, Each
NDIEC-886	Protein Loading Buffer BLUE (2X), Each